In order to improving the top-design level and the development probability of military information system, put forward a requirement analysis method of military information system based on DOD2.0. Based on analyze DOD2.0 system frame, aim at the capability requirement, operational requirement, services requirement, systems requirement, procure requirement and standards requirement, design a requirement description framework of military information system. Research the requirement description method and the requirement description process emphatically. Results show that the method is scientific and easy to use. Project viewpoint has great significance in military information system requirement engineering field.