Aiming at the mobile robot trajectory control problem, propose a method of fuzzy control algorithm for robot trajectory control. Adopt perceived deviation angle of robot with electro-optical sensors, select Mamdani model to design fuzzy controller, acquire the robot walking directions and rectification of the size, and realize accurate guidance. And simulate fuzzy controller based on Matlab. The simulation and application result shows that the controller can improves the accuracy of robot tracking control to enable stable mobile robots found white lines moving quickly.