Aiming at the detection problem of twilight target in complex background, A new method of twilight target detection was proposed in this article. The algorithm work as the following step: firstly, do row mean removing preprocessing operation to remove the clutter of background based on the strong correlation between adjacent lines; then, locating the target by search the maximum on the result image of preprocessing. Experiments on real world video shows the proposed method can robustly detect the twilight aerial target in real-time.