In order to tackle the problems as the uncertainty of underwater environment, the strategy debugging and so on, a referee auto-control system of robot water polo game simulator has been designed in the study. The paper introduces the design framework, including the position and direction of the underwater robot through modeling the kinematics model of underwater robot, movement control simulation, modes of disturbed motion simulation, collision treatment simulation and automatic control in the process of robot water polo game simulator simulation etc. The design framework also supplies the correlational control interfaces of the entity of underwater robot polo competition and its expanding functions. The result shows that the system can provide effective control and management measures for foul situations, game process control, goal judge, game special rules and so on, which appear in the process of robot water polo game simulator. Beside that, the system can extend to be applied not only into other items in robot water polo game simulator, but also into 3D simulation and the entity of underwater robot polo competitions.