A kind of vibration isolation and buffering system is designed to solve the problem of vibration isolation and buffering of wheeled vehicle structure and to protect the safety of equipment during transportation. The system consists of a stable platform, a vibration isolation system chassis, a vibration isolator, a stabilizer and a buffer limiter; When the equipment is transported, the lifting mechanism together with the chassis of the vibration isolation system descends to the flat plate of the frame, the chassis of the vibration isolation system is tightly pressed on the frame by the pressure of the lifting mechanism, the locking between the platform and the chassis of the vibration isolation system is loosened, at this time, the stable platform and the equipment are borne by the vibration isolation system, and the equipment is in a flexible supporting state; The vibration isolator plays the role of vibration isolation and buffering when the vehicle is running. When the vehicle starts, brakes, turns and is impacted, the buffer limiter and stabilizer play the role of stabilizing and limiting. The simulation results show that the stiffness, strength and performance characteristics of the system meet the requirements of the safe transportation of the equipment, and the reliability and supportability can also be guaranteed, which meets the requirements of the development of the vibration isolation and buffering system.