Aiming at the problem that most of the current research on UAV route planning focuses on the improvement of the algorithm, and there is a lack of three-dimensional visual display of the planning results, a simulation platform for UAV three-dimensional route planning based on Matlab software is created. Using the App designer function of Matlab software to complete the development of graphical user interface, establish the map model based on terrain threat, use a variety of common swarm intelligence algorithms in the market at present, compare the differences between various swarm intelligence algorithms intuitively, and add algorithms according to the needs to compare with the existing algorithms. The design platform can realize 3D route planning including multiple swarm intelligence algorithms, which is used to verify the feasibility and reliability of the algorithm, and intuitively reflect the advantages of the new algorithm. Through the actual operation of the platform, the results show that the platform has the ability of three-dimensional visual display of the results of the route planning algorithm.