Aiming at the problem of rolling bearing fault on-line monitoring, the fault diagnosis is carried out by combining LabVIEW and Matlab programming methods. This paper discusses the fault signal characteristics of rolling bearings, introduces the kurtosis analysis method and resonance demodulation method, and realizes the algorithm by using LabVIEW and Matlab joint programming. The analysis method is verified by the experimental data of typical bearing faults, and the results show that the system can effectively analyze and identify the characteristic faults of bearings, and can be used for bearing fault monitoring.