According to the special application environment characteristics of missile borne storage test device and the requirements of internal trigger, a design method of internal trigger circuit is proposed, which takes the accelerometer ADXL78 with zero frequency and the high-speed voltage comparator LM311 as the core. The trigger circuit module is designed and implemented, which has been verified and applied by static detection and dynamic detection in the laboratory. The results show that the trigger circuit meets the requirements, the trigger threshold is flexible and adjustable, it can trigger the missile borne storage test device stably and reliably, and it can provide the necessary trigger guarantee for the engineering application of the missile borne storage test device.