Aiming at the dynamic calibration requirements of carrier-based aircraft ground base optical landing system, the dynamic flight test system based on unmanned aerial vehicle (UAV) platform has been built, which is using the carrier phase difference of global navigation satellite system (GNSS). Firstly, calibration requirement of the Fresnel lens optical landing system (FLOLS) has been analyzed, and the technical specifications requirement of the dynamic fly-checking system has also been confirmed. Then, the dynamic calibration flight test system has been constructed, which is consist of UAV platform, high-definition camera and recording module, airborne differential GNSS module, attitude measurement module and ground display and control terminal. Finally, the dynamic calibration process has been introduced. The coordinate of geometric center of the Fresnel lamp are obtained by eccentric observation method, and the position information of imaging geometric center of aerial camera is obtained by combining the attitude information of IMU module. The application results show that the system can provide reference for dynamic calibration of FLOLS.